Anti-CCD absorbent is an additional reagent designed for the incubation with blot-based allergy profiles. The absorbent eliminates anti-CCD IgE antibodies from patient serum, which increases the specificity of test results if these antibodies are present in the sample.
Anti-CCD IgE antibodies are directed against sugar structures of proteins and can be detected in around 25 % of allergy patients as well as in non-allergic individuals. They generally have no clinical relevance.
In extract-based in vitro allergy diagnostics, these antibodies complicate the interpretation of positive results because the following cases cannot be differentiated:
- positive reaction caused by IgE antibodies against peptide epitopes
- positive reaction caused by anti-CCD IgE antibodies
- positive reaction caused by a combination of the two antibody types
Differentiation between the described reactions is possible if the absorbent is used and the anti-CCD IgE antibodies are eliminated.
The anti-CCD absorbent is useful if the patient sample demonstrably contains IgE antibodies against CCD structures. This is indicated by a positive CCD band on the incubated allergy profile. In this case, the serum should be retested using the anti-CCD absorbent.
The patient sample is incubated with anti-CCD absorbent for 60 minutes at room temperature according to the instruction provided with the reagent. The sample can then be used directly in the incubation with the respective allergy profile.